Bioinformatics and Functional Genomics

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Chapter 7: Gene expression: microarray data analysis


Web resources from Chapter 7
Website URL
The Microarray Gene Expression Data (MGED) Society http://www.mged.org
Terrence Speed laboratory http://www.stat.Berkeley.EDU/users/terry/zarray/Html/papersindex.html
HuGE http://www.HugeIndex.org
Stanford Microarray Database http://genome-www5.stanford.edu/MicroArray/SMD/
SNOMAD http://www.snomad.org
GeneTraffic software from Iobion http://www.iobion.com
Partek Pro http://www.partek.com
GeneSpring http://www.sigenetics.com
GeneSight and Genedirector http://www.biodiscovery.com
Spotfire http://spotfire.com
SAM http://www-stat.stanford.edu/~tibs/SAM
Cluster http://rana.lbl.gov/EisenSoftware.htm

 

Tables

Table 7-1. Log ratios of gene expression values are often easier to interpret than raw ratios.
Time (t) Behavior of gene Raw ratio value Log2 ratio value
t=0 Basal level of expression 1 0
t=1 No change 1 0
t=2 Two-fold up-regulation 2 1
t=3 Two-fold down-regulation 0.5 -1

 

Table 7-2. Test statistics for microarray data. Adapted from Motulsky (1995) and Zolman (1993).
Paradigm Parametric test Nonparametric test
Compare one group to a hypothetical value One-sample t-test Wilcoxon test
Compare two unpaired groups Unpaired t-test Mann-Whitney test
Compare two paired groups Paired t-test Wilcoxon test
Compare three or more unmatched groups One-way ANOVA Kruskal-Wallis test
Compare three or more matched groups Repeated-measures ANOVA Friedman test

 

Table 7-3. Resources for microarray data analysis
Resource Comment
Y.F. Leung’s microarray site  
Stanford Microarray Database Includes lists of software tools
TIGR Various programs
Microarray & Data Analysis Literature on array analysis
MadB National Cancer Institute and CIT, NIH

 


Table 7-4. Microarray data analysis and general statistics packages for the analysis of microarray data. Dozens of other packages are available.
Software Company/organization
Access Microsoft
Array Suite Affymetrix
Array Organizing Tool (ARROGANT) The University of Texas Southwestern Medical Center at Dallas
Atlas Navigator Clontech
BioArray Software Environment (BASE)  
BioMine  
Cleaver Stanford University
Cluster Analysis of Gene Expression Dynamics (CAGED) Harvard Medical School
Cluster/Treeview Stanford University
DRAGON Kennedy Krieger Institute
Excel Microsoft
Expression Profiler European Bioinformatics Institute
GeneCluster Whitehead Institute
GeneCruiser Whitehead Institute
GeneSight BioDiscovery
GeneSpring Silicon Genetics
GeneTraffic  
GeneLinker Molecular Mining
GeneViz  
J-Express MolMine
Partek Partek
Pathways Research Genetics
S-PLUS Insightful Corp.
Signficance Analysis of Microarrays (SAM) Excel plug-in
SNOMAD Kennedy Krieger Institute
Spotfire  
SPSS-Clementine  
STATA  
STATISTICA  
TIGR  
X-MINER  
XLSTAT Excel plug-in
 
 
 
 

 

 

 
 

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