Additional Table 4. Primer sequences and other information of the quantitative real-time PCR experiments. For the other genes whose primer sequences are not listed here, real-time PCR were carried out using pre-designed TaqMan® Gene Expression Assays (Applied Biosystems). Information on these primers and assays can be found at www.appliedbiosystems.com.

 

 

 Gene                           Primer Sequence                           Product  Size        System

 

ATP 5O    F : 5’-GCTTGCTGAAAATGGTCGAT-3’                     336              LightCycler

                 R : 5’-TCACTGATGGCAGAAAACCA-3’                                        ABI (7900)

 

APT 5J     F : 5’-CATTTGCGGAGGAACATTG-3’                       315              LightCycler

                 R : 5’-CAACTAATCCGTGACAAATTACCAG-3’                            ABI (7900)

 

PTTG1     F : 5’-TGCTGCCTAAGGAAGTTTGG-3’                     342              LightCycler

                R : 5’-ACAAATGCCCAAGAGGTCAG-3’                                         ABI (7900)

 

USP16     F : 5’-TTGAATGCTGCTCTTCATCC-3’                      307               LightCycler

                R : 5’-GCTTTTTGGCATTGGTGTAA-3’                                           ABI (7900)

 

ZNF294   F : 5’-CACGTCTCTAGTTGCTGGGCT-3’                   301              ABI (7900)

                R : 5’-ACGTTCTCCAAGTTGTGAGCTG-3’

 

DSCR3    F : 5’-GCCACGGAGATTCAGAACATTC-3’                301              ABI (7900)

                R : 5’-ATGCCGCTGGCTGTAGCT-3’